Fig. 7. Evaluation of other vesicular trafficking proteins as possible marker of carriers of the minor regulated pathway. Parotid tissue was processed for immunofluorescence without (Ctl) or with (Cch) a 3 minute treatment with 40 nM CCh. Immunofluorescent specimens have been counterstained with BODIPY-phallacidin (green) for actin in each case. (A) VAMP2 (red) staining of control tissue; (B) VAMP2 (red) staining of stimulated tissue; (C) Rab11 (red) staining of control tissue; (D) Rab11 (red) staining of stimulated tissue. Solid arrowheads identify prospective subapical foci of VAMP2 and Rab 11, and open arrowheads identify some of the foci that have relocated to the luminal side of the actin band. Bar, 10 µm.