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Fig. 1. ATP measurements with the luciferase assay. (A) Calibration curve showing that the assay is linear. (B) The calculated intracellular ATP concentrations after various times of ATP depletion. Data were pooled from four independent experiments. The line represents a sum of two exponential functions: [ATP]=2111 µMxe-t/3.5+250 µMxe-t/27. This function is used to calculate the intracellular ATP concentration at any given time of ATP depletion.