Fig. 2. Ca²⁺-induced exocytosis after ATP depletion. Representative patch-clamp capacitance traces in control conditions, using pipette solutions containing 1 mM ATP, and after various times of incubation in an ATP-depleting medium using ATP-free pipette solutions. The incubation times until patch rupture in minutes are indicated next to the traces. In A pipette solutions contained 10 µM Ca²⁺ and in B 300 µM Ca²⁺. Time is given from the time of patch rupture. Initial capacitances have been subtracted for clarity. (C) Initial capacitance (C_i) versus time of ATP depletion with linear regression C_i(t)=0.0008 pF/minutext+2.692 pF.