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Fig. 6. Ribosome export mutants identified by screening ts- mutant libraries. (A) 5' ITS1 localization in rat2-1/nup120 (i) grown at 25°C and in wild type (h), rat7-1/nup159 (j), nsp1(L697P) (k), gle2(N273K,D290N) (l), yrb1(F191S) (m), and prp20(S297N) (n) mutants shifted to 37°C for 1 hour. Chromosomal DNA was labeled with DAPI (a-g). Arrows point to DAPI-stained chromatin while arrowheads point to the nucleolus. (B) Pre-rRNA processing in wild-type (lanes 1-3 and 7-9), rat7-1/nup159 (lanes 4-6), nsp1(L697P) (lanes 10-12), gle2(N273K,D290N) (lanes 13-15), and yrb1(F191S) (lanes 16-18) mutants shifted to 37°C for 1 hour. Cells were pulse labeled for 3 minutes (lanes 1-6) or 1 minute (lanes 7-18). Pulse and chase times are indicated above each lane. Sizes of the rRNAs are indicated.