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Fig. 4. (A) Fluorescent micrographs illustrating the effect of transportin on the nuclear uptake of GST/GFP/M9 (wt or mt) fusion proteins. The micrographs were taken 60 minutes after the injection of GST/GFP/M9wt alone, GST/GFP/M9mt plus transportin or GST/GFP/M9wt plus transportin. N, nucleus; CV, contractile vacuole. (B) The nuclear uptake kinetics, expressed as N/C fluorescent ratios, are shown for amoebae injected with GST/GFP/M9wt alone (—{diamondsuit}—), GST/GFP/M9mt plus transportin (—{blacktriangleup}—), GST/GFP/M9wt plus transportin (—{blacksquare}—), or fluorescein-labeled BSA (...[UNK]...), which served as a background control. Respectively, 13, 6, 17 and 7 cells were analyzed.