Fig. 6. CD-induced PAI-1 expression is sensitive to tyrosine kinase inhibitors. Quiescent R22 cultures were pretreated for 30 minutes with progressively increasing concentrations of genistein (A) or PPI (B) prior to CD treatment. RNA was isolated, and northern blots were probed for PAI-1 and A50 transcripts. Representative blots are shown in A and B. —/— refers to DMSO vehicle only. The corresponding histograms illustrate PAI-1 mRNA abundance normalized to A50 signal for replicate experiments (mean±s.d.). Concentrations of genistine and PP1 (>25 µM and 10 nM, respectively) effectively ablated PAI-1 transcript expression.