Fig. 6. Turn-over of myotubularin. COS cells transfected with wild-type myotubularin were labeled with [³⁵S]methionine/cysteine and cold chased for 0, 15 or 30 minutes and 1, 2, 3 or 5 hours. Myotubularin was immunoprecipitated with the 1G1 monoclonal antibody. The period of cold chase is indicated above the lanes. Myotubularin is indicated by an arrow. Above, an additional protein is trapped probably by the beads as it is also present in the first lane without immunoprecipitant antibody. The estimated half-life is about 4 hours.