(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 7. Effect of myotubularin on endocytic markers and PtdIns3P distribution. (A) COS cells transfected with wild-type myotubularin do not show changes in endocytic markers such as dynamin (clathrin-coated vesicles), caveolin 1 (uncoated vesicles), and Rab5 (endosomes). Note the strong labeling of a membrane ruffle with anti-myotubularin antibody in the caveolin 1 co-staining. (B) COS cells co-transfected with wild-type or C-ter delSID myotubularin constructs and a myc-tagged 2XFYVE expression construct show no co-localization and no effect of myotubularin with the endosomal staining by 2XFYVE.