Fig. 7. In vivo changes in the subcellular localization of the linker-GFP construct upon cholesterol depletion using ß-methyl cyclodextrin and changes in the subcellular localization of various GFP chimeras upon incubation with 2-bromopalmitate. COS-7 cells were transfected with the linker-GFP plasmid and incubated with 10 mM ß-methyl cyclodextrin for 0, 10, 20 or 30 minutes (A-D) at 36 hours after transfection. Arrows in D denote changes in the plasma membrane fluorescence. COS-7 cells were also transfected with the linker-GFP, G2A-GFP, C3S-GFP, C3S/S9C-GFP, C3S/S15C-GFP or C3S/S21C-GFP plasmids and, 36 hours post-transfection, incubated with 25 µM 2-bromopalmitate for 16 hours (E-J). Bar, 50 µm.