Fig. 6. Expression of p53 and p53-related gene products in fibroblasts from non-AD and AD patients: western blot analysis. (A) Protein extracts from two non-AD (lanes 1-2 and 3-4) and two AD (lanes 5-6 and 7-8) fibroblast cultures in basal conditions (lanes 1, 3, 5 and 7) and 2 hours after the H₂O₂ pulse (lanes 2, 4, 6 and 8) were processed with specific antibodies against p53, p21, bax, GADD45 and ß-tubulin, as indicated in the method section. (B) Data obtained in fibroblasts from six non-AD and six AD patients in basal conditions (open bars) and after H₂O₂ pulse (black bars) were calculated by densitometric analysis and normalized as a percentage of basal expression. Values are expressed as means±s.e.m. of at least three separate experiments for each sample. ^*P<0.01 versus the corresponding basal values.