Fig. 7. Phosphorylation status of p53 in fibroblasts from non-AD and AD patients: an immunoprecipitation study. Protein extracts from one non-AD (lanes 1-2) and three AD (lanes 2-4, 5-6 and 7-8) fibroblast cultures in basal conditions (lane 1, 3, 5 and 7) and 2 hours after the H₂O₂ pulse (lane 2, 4, 6 and 8) were immunoprecipitated with antibodies against p53 and immunoblotted with specific antibodies against phospho ser15, phospho ser392 or p53, as indicated.