Fig. 2. Phenotypes of RHO5Q91H overexpression. Cells were grown on glucose-containing medium and streaked onto plates containing 2% galactose to induce transcription of the mutated protein. Plates were incubated at the temperatures indicated for 3-4 days. Strain DHD5 was used as a host in this experiment. The construction of the mutant is described in Materials and Methods. pGALrho5^*, strain carrying the activated RHO5Q91H allele; wt, wildtype.