Fig. 6. Influence of Rho5p on actin dynamics. (A) Effect of a rho5 deletion on the distribution of actin upon heat shock. Cells were investigated at the times indicated (for details, see Materials and Methods). Strains employed were HSH1-1C and HSH1-4A. (B) Effect of RHO5Q91H overexpression on actin kinetics. Strain DHD5 was transformed with pJJH447 (vector not carrying any RHO5 sequences) or pSH8 (pGALrho5^*; vector carrying the activated RHO5Q91H allele). Yeast cells were grown in glucose-containing minimal medium overnight. Then cells were inoculated into 50 ml of fresh minimal medium containing the carbon source indicated and grown for about 4 hours to an optical density of between 0.3 and 0.6 at 30°C. To observe actin dynamics under heat shock the cultures were shifted to 37°C, and actin distribution after heat shock was investigated at the times indicated using the staining procedure described in Materials and Methods.