Fig. 5. Localization and translocation of endogenous S100A13 in endothelial cells (ECV). (A) S100A13 protein was mainly localized in the nucleus of the untreated cells. Similar nuclear localization of S100A13 was also observed in cells treated with brefeldin A (C) and bafilomycin A (E) before angiotensin II stimulation. By contrast, small vesicles at the cellular periphery (indicated by arrows) were evident after angiotensin II stimulation (B); and treatment with amlexanox (D) or demecolcine (F) before addition of angiotensin II had no effect on the translocation of the protein. Areas with a series of vesicles were enlarged by 20% and presented in the small squares at the right hand corners (B,D,F). The samples were analyzed by confocal microscopy. Bar, 15 µm (F).