Fig. 6. Localization and translocation of endogenous S100A6. (A) S100A6 was mainly distributed around the cell nuclei and in the cytoplasm of ECV cells; S100A6 translocated in the form of vesicles (indicated by arrows) in cells stimulated by angiotensin II (C); (B) Fewer cells with vesicle formation were observed after amlexanox treatment. However, S100A6 translocation in angiotensin-II-stimulated cells was not affected by brefeldin A (D) nor by demecolcine (E) or bafilomycin A (F). All the samples were analyzed by confocal microscopy. Bar, 15 µm.