Fig. 7. Effects of the inhibitory drugs used to study the various translocation pathways. Cells were left untreated (A,C,E), incubated with brefeldin A (B), amlexanox (D) or demecolcin (F) and stained with mouse anti-ß-COP (A,B) and mouse anti-tubulin (E,F) antibodies. Phalloidin-TRITC was used to localize actin stress fibers (C,D). The scale bar in B applies for both A and B; the scale bar in D applies for C and D; and the scale bar in F applies for E and F. All the scale bars are 15 µm.