Fig. 8. Quantification of S100 protein translocation. Cells producing vesicles containing either S100A13 or S100A6 were counted and divided by the total cell counts (approximately 200 cells were counted for each experiment), and the columns represent the mean of three experiments. (A) Percentage of cells with S100A13 translocating in vesicles in response to angiotensin II alone or after treatment with the drugs indicated. ^* indicates a statistically significant difference between cells stimulated with angiotensin II alone and treated with brefeldin A or bafilomycin A (P<0.05). (B) Percentage of cells with S100A6 translocating in vesicles in response to angiotensin II and the drugs indicated. ^* indicates a statistically significant difference between cells treated with angiotensin II alone and with amlexanox (P<0.1). The P value was calculated by using Student-t-variant R. A. Fisher and F. Yates.