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Fig. 6. Suppression of DNA replication by EcoRI-treatment. Demembranated Xenopus sperm nuclei were incubated for 90 minutes at 23°C in the extract with or without EcoRI, caffeine and geminin in the presence of [{alpha}-32P]dCTP. The extent of DNA synthesis is represented as a percentage of accumulative DNA synthesis after a 90 minute incubation in the absence of DNA damaging agents. 100% DNA synthesis is estimated to be an amount equivalent to the duplication of about 90% of input DNA.