Fig. 8. EcoRI-induced DNA synthesis was co-localized with RPA foci and insensitive to caffeine treatment. Demembranated Xenopus sperm nuclei were incubated in the extract containing geminin and biotin-dUTP for 30 minutes at 23°C in the absence (a-c), or presence of EcoRI (d-i) or EcoRI+caffeine (j-o). Localization of biotin-dUTP (a,d,g,j,m) and RPA (b,e,h,k,n) is shown in green and red, respectively. Merged images are also shown in panels c, f, i, l and o. Panels g, h, i, m, n and o are enlarged images of the nuclei indicated by arrowheads in panels d, e, f, j, k and l, respectively. Bars, 50 µm (a-f,j-l); 20 µm (g-i,m-o).