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Fig. 1. Composite image of single-stage RT-PCR analysis of human tissue, embryo and cell line RNA using occludin primers shown in Table 1. Primers within the C-terminus (H5/H6) amplify a single product corresponding to the published occludin sequence, while primers flanking the TM4 and immediate proximal C-terminus region (H1/H2 and H3/H4) amplify two products, the upper corresponding to the published cDNA sequence while, in the lower band, the TM4 and immediate 3'-flanking sequence are deleted. B, embryo blastocyst stage; BE, bronchial epithelium; C, cervix; Ca, Caco-2 cells; HBE, 16HBE cells; HuV, HuVEC cells; K, kidney; M, marker lane; Mo, embryo morula stage; U, uterus.