Fig. 4. Expression analysis of NUANCE. (A) A northern dot-blot of a variety of human tissues and cell lines, probed with a fragment corresponding to the ABD of NUANCE. These results are summarized in Table 2. Lane 12 represents controls: A12, yeast total RNA; B12, yeast tRNA; C12, E. coli rRNA; D12, E. coli DNA; E12, poly(A); F12 human C₀t_1 DNA; G12, human DNA 100 ng; H12, human DNA 500 ng. (B) Immunoblots of the COS7 cells homogenate. (C) Cells fractionated into nuclei, cytosol and cytoplasmic membranes. Each fraction was separated on 3-15% gradient SDS-PAGE and immunoblotted with anti-NUANCE, anti-lamin B and anti-annexin A7 mAbs as indicated. The positions of molecular mass marker proteins are shown on the left.