Fig. 5. Nuclear localization of NUANCE in COS7 cells (C,G) immunolabeled with the anti-NUANCE mAb K20-478. NUANCE remains associated with NE during prophase (A,B, arrow) and with chromosomes during prometaphase (A,B, large arrowhead). At later stages NUANCE is diffusely distributed throughout the cytoplasm (A, B, small arrowhead). NUANCE is also detected in bridges, which were occasionally seen between the nuclei of two cells (C,D, arrow). DNA was visualized with DAPI (B,D). Bar, 20 µm.