Fig. 7. Effect of LatA treatment on the subcellular distribution of endogenous NUANCE. COS7 cells were incubated in the absence (A-C) and presence (D-I) of 1 µM LatA for 30 minutes (D-F) and 60 minutes (G-I). (J-L) COS7 cells were stimulated to migrate by wounding a confluent monolayer of cells. 6 hours after wounding, the cells were processed for immunofluorescence microscopy. Cells were stained with an anti-NUANCE mAb followed by an Alexa-488-conjugated secondary antibody (A,D,G,J) and TRITC-phalloidin (B,E,H,K) to visualize actin filaments. (C-L) overlays. Bars, 20 µM.