Fig. 1. Assembly and disassembly of arsenite-induced stress granules. DU145 cells were cultured in the absence (control) or presence of arsenite (1 mM) for 30 minutes (STRESS), washed and allowed to recover for 1 hour or 3 hours (RECOVERY) before processing for two-color immunofluorescent microscopy. TIA-1 protein is identified using a polyclonal antibody (green). Poly(A)+ RNA is revealed by in situ hybridation using an oligo-dT probe (red). Sites of co-localization of TIA-1 and poly(A)+ RNA appear yellow. Nuclei are counterstained using Hoechst dye (blue).