Fig. 8. Neural progenitors and differentiated astrocytes express Cx43. Single cell suspensions of striatal progenitors were expanded for 10 days in the presence of EGF to form floating neurospheres, which were fixed, sectioned and immunolabeled with a monoclonal antibody raised against Cx43 (B). The image corresponds to the merging of 10 planes acquired by confocal microscopy. Virtually all cells in the neurosphere were positive for Cx43. The corresponding Nomarski view is shown in A. Bar, 75 µm. (C) Neurospheres were allowed to differentiate for 8 days and were double-labeled with a polyclonal antibody against Cx43 (red) and a monoclonal antibody anti-GFAP (green). Cx43 was detected in GFAP-positive cells. Bar, 10 µm.