Fig. 1. Molecular analysis of Stau2 isoforms. (A) Alternative splicing in the 5' end of the Stau2 gene. Alternative splicing generates two transcripts, T1 and T2, that differ by the insertion of 131 nucleotides in T1 compared with T2. Arrows indicate the first in frame ATG initiation codon for translation of Stau2⁶² and Stau2^59/52, respectively. Note that the two ATG are not in frame in the T1 transcript and therefore translation initiation of Stau2⁶² starts at the second ATG. ^* indicates the presence of stop codons in the three open reading frames. (B) Amino-acid sequence alignment of Stau2 dsRBDs and the consensus dsRBD sequence. (C) Alignment of the amino-acid sequence of mouse Stau2⁶² (62), Stau2⁵⁹ (59), Stau2⁵² (52), mouse Stau1⁵⁵ (St1) and Drosophila Staufen (Dro). The sequence of Stau2⁵² is from Buchner et al. (Buchner et al., 1999). The position of dsRBDs (dsRBD1 to dsRBD5) is indicated above the sequence. Dots indicate amino-acid residues identical to those of Stau2. All identical amino-acid residues are boxed. Dashes represent gaps in the amino-acid sequences. These sequence data have been submitted to the DDBJ/EMBL/GenBank databases under accession numbers AF459099 and AF459100.