Fig. 5. Stau2 is found in dendrites but not in axons. (A) Hippocampal neurons in culture were fixed and labeled with rabbit anti-Stau2 (green) or mouse monoclonal anti-MAP2 (red) antibodies. Arrows indicate axons (negative in MAP2) with little or no staining for Stau2. The arrowhead indicates a very thin dendrite (MAP2 and Stau2 positive) comparable in size to the marked axon. (B) Hippocampal neurons in culture were fixed and labeled with rabbit anti-Stau2 (green) or mouse monoclonal anti-tau1 (red) antibodies. Arrows indicate axons (negative for Stau2). In (A,B), the upper left panels show phase contrast microscopy. The lower right panels show superposition of both red and green signals. The average diameter of the cell body of a typical CA1 pyramidal neuron is between 8 and 12 µm.