Fig. 7. Flow cytometric analysis of the annexin-6-expressing transfectant. (A) One million stable transfectants with the annexin 6 cDNA (Anx#1) or mock vector (Mock#1) were labeled with various antibodies. Anti-FAK antibody (1:200 dilution) was used as a negative control. Anti-annexin 6 and anti-ß1-integrin polyclonal antibodies were used at the final dilutions of 1:500 and 1:200, respectively. (B) Anx#1 was also labeled with two different monoclonal (ZYMED and Transduction Laboratories) and one polyclonal antibodies (Santa Cruz) to annexin 4 at the final dilutions of 1:200. The source of the anti-annexin 4 antibody is indicated in brackets. Propidium iodide was used for the detection of any damaged or leaky cells. Gates for propidium iodide staining and FITC intensity were placed on the basis of values of negative cells. Numbers in quadrants represent the percentage of gated cells.