Fig. 1. A schematic representation of the GAL4-hDaxx deletion mutants, protein expression and cellular localization. The GAL4-DNA-binding domain and the domains of hDaxx are indicated at the top. The GAL4-DNA-binding domain is represented by the black box. The paired amphipathic helices (PAH1 and PAH2) are represented by the dark gray boxes. The acid-rich domain (AD) is represented by the light gray box and the Ser/Pro/Thr-rich domain (SPT) is represented by the medium gray box. A solid line represents regions of the proteins that have been deleted. Protein was present (+), absent (-) or drastically reduced, as determined by western blot analysis. The cellular localization of each deletion mutant was determined by immunofluorescence using the mouse monoclonal anti-GAL4 antibody. Cellular localization for each mutant is presented as either diffuse throughout the cell (D) or strictly nuclear (N).