Fig. 5. Morphological analysis of the degree of maturation of cell-cell contacts in T98G and U373MG cell lines. Confocal analysis of immunofluorescence staining. The glioblastoma cell lines were plated onto poly-L-lysine-coated glass coverslips, fixed in 4% paraformaldehyde and double-stained for ß-catenin and the indicated antibodies or the FITC-conjugated phalloidin to stain actin. The arrowheads indicate mature cell-cell contacts, and the arrows indicate immature cell-cell contacts. Bar, 10 µm.