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Fig. 1. Clone VLP27 encodes a novel microtubule-binding protein, MIR1. (A) The clone VLP27 (GFP-MIR1) was expressed at medium or low levels in BHK cells (first and third panel). The same cells were also stained with the tubulin antibody M1{alpha} (second and fourth panel). The magnification was 63x for the left two panels and 100x for the right ones. The arrows point to MIR1 staining of individual microtubules. (B) The diagram of the homology between MIR1 and its closest relative MID1/midin. The domain configuration is identical, except for the added P-stretch region and the missing N-terminal RING finger and B-Boxes in MIR1. (C) Northern blot of various human tissues probed against MIR1 RNA. (D) Immunoblot analysis of post-nuclear lysates from selected cells using the affinity-purified antibody Ab284 directed against MIR1. Lysate from BHK cells expressing untagged MIR1 was analyzed in parallel.