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Fig. 3. Overexpressed MIR1 localizes to newly polymerized microtubules. BHK cells transfected with GFP-MIR1 were treated with nocadozole to depolymerize the microtubules. The drug was washed out and the microtubules allowed to repolymerize for the indicated times. The cells were fixed and analyzed for MIR1 by GFP fluorescence (left column) and for tubulin using the M1{alpha} antibody (right column). Arrows highlight transfected cells with newly formed microtubules.