Fig. 1. The C-terminal 630 amino acids of Cenp-F localises to kinetochores. (A) Schematic of Cenp-F showing the domain encoded by C630 and the fragment used to generate the anti-Cenp-F antibody, SCF.1. (B) HeLa cells stained to detect endogenous BubR1 (red), myc-tagged C630 (green) and DNA (blue). During interphase C630 localises to the nucleus and in prometaphase it co-localises with BubR1 at kinetochores. Bars, 5 µm. (C) Immunoblot of pellets (P) and supernatants (S) following extraction of interphase and mitotic HeLa cells showing that SCF.1 recognises a single band significantly greater than 200 kDa.