Fig. 7. The CAAX motif of Cenp-F is required for nuclear envelope and kinetochore localisation. (A) HeLa cells stained to detect myc-tagged proteins, Cenp-F and DNA as indicated. Nuclear envelope and kinetochore localisation of Cenp-F is diminished in the presence of C630 but is readily apparent when the C:S mutant is expressed. (B) Bar graph quantitating the number of cells with Cenp-F detectable at the nuclear envelope 12 hours after release from a G1/S block. Nuclear envelope staining is less frequent in cells expressing C630. (C) Projections of deconvolved image stacks showing HeLa cells stained to detect myc-tagged proteins (red), Bub1 (green) and DNA (blue). The C:S and V:D mutants localise poorly to kinetochores.