Fig. 8. Farnesyl transferase activity is required for Cenp-F localisation. (A) The percentage of cells with Cenp-F at the nuclear envelope in the presence or absence of SCH 66336 at various times following release from a G1/S block. (B) Early prophase HeLa cells cultured in the presence or absence of SCH 66336 stained for Cenp-F (green), lamin B2 (red) and DNA (blue) showing that nuclear envelope localisation of Cenp-F is not apparent in the presence of the FTI. (C) Nocodazole-arrested mitotic HeLa cells cultured in the presence or absence of SCH 66336 were isolated by shake off, centrifuged onto glass slides and then stained for Cenp-F (green) and phospho-histone H3 (red). Kinetochore staining is not apparent in the presence of SCH 66336.