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Fig. 6. Effects of calpain inhibition on RFP-zyxin and GFP—{alpha}-actinin localization in CHOK1 cells. (A) Images from control cells (a-f) or cells treated with ALLN (g-i). Red shows zyxin and green shows {alpha}-actinin; areas of co-localization appear as yellow. Zyxin showed decreased localization with {alpha}-actininin in cells treated with ALLN. (B) Quantification of {alpha}-actinin and zyxin co-localization in control cells or cells treated with ALLN from five separate experiments (P<0.01). Bars, 10 µM.