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Fig. 7. Effects of calpain inhibition on the composition of zyxin-containing focal complexes. (A) CHOK1 cells co-transfected with GFP—{alpha}5-integrin and RFP-zyxin were treated with (d-f) or without (a-c) ALLN and fixed. Zyxin-containing focal complexes contained GFP—{alpha}5-integrin in both control and calpain-inhibited cells. (B) Cells transfected with zyxin-RFP were treated with (d-f) or without (a-c) ALLN and fixed and stained. Zyxin-containing focal complexes in both control and ALLN-treated cells contained vinculin. Bars, 20 µm.