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Fig. 8. Effects of calpain inhibition on RFP-zyxin and GFP—{alpha}-actinin localization and focal complex disassembly. Video sequences of magnified regions of control cells (a-1) or cells treated with ALLN (m-r). Red shows zyxin and green shows {alpha}-actinin; areas of co-localization appear as yellow. Images were collected at t=0, 6, 12, 18, 24 and 30 minutes. White arrows show zyxin-containing focal complexes that disperse after {alpha}-actinin localization. Gray arrows show zyxin-containing focal complexes that translocate to the cell center. Open arrows show stabilized zyxin-containing focal complexes that did not co-localize with {alpha}-actinin, and did not translocate or disperse in cells treated with ALLN (Movies 9-11). Bars, 20 µm.