Fig. 2. TIF1ß is targeted to centromeric heterochromatin in ES-derived neurons. A and D show representative phase-contrast photomicrographs of undifferentiated ES cells growing on a layer of feeder cells in the presence of LIF (control) and ES-derived neurons taken 4 days after plating of the RA-treated embryoid bodies in defined medium (RA), respectively. Confocal images of single optical sections through the nucleus of undifferentiated and differentiated cells are shown. B and E show the Hoechst DNA staining, and C and F, the TIF1ß staining. Bars, 100 µm for (A) and (D) or 5 µm for others.