Fig. 3. Analyses of the mRNA expression levels of hLTBP-3 and hL3+EGF splice variant in various human tissues and cell lines. (A) Human multi tissue northern blot was hybridized with a radioactively labeled cDNA fragment of hLTBP-3. A single 4.6 kb mRNA species corresponding to human LTBP-3 was detected. Hybridization to human ß-actin was used to control the loading of RNA. (B) Total RNA was extracted from various human cell lines, separated on a formaldehyde-agarose gel and immobilized on a nylon filter. Northern hybridization analysis was carried out using the hLTBP-3 cDNA probe. The lower panel shows the hybridization signals of glyceraldehyde-3-phosphate dehydrogenase (G3PDH), which were used to control the RNA loading. (C) RT-PCR and Southern hybridization analyses of cDNA prepared from different human tissues using primers specific for hL3+EGF splice variant. The expected amplification product migrated at 154 bp. The lower panel illustrates RT-PCR amplification of an abundantly expressed G3PDH in the corresponding tissues.