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Fig. 2. PKA phosphorylates PCTAIRE-1. (A) Elution profile of labeled tryptic peptides of PCTAIRE-1, prepared as described in Materials and Methods. (B) Wild-type, or mutated recombinant PCTAIRE-1 proteins were tested as PKA substrates using 1 U of purified PKA. (top panel) Coomassie-blue-stained gel; (bottom panel) autoradiograph at the position of PCTAIRE-1.