Fig. 7. Morphological effects of PCTAIRE-1-YFP constructs transfected into Neuro-2A cells suggest a role of PCTAIRE-1 in the prevention of neurite outgrowth. (A) Indicated constructs were transfected into Neuro-2A cells. Two days after transfection, the individual wells were scored for green cells that had neurites longer than twice the length of the cell body. The cells were then treated with 10 µM forskolin, left for 24 hours, and scored again. 5-10 fields were counted totalling 500-1000 transfected cells. The experiment was repeated twice with similar results. (B) Images of cells transfected with YFP, or wild-type, kinase-dead, or the double mutant S119/S153-YFP-PCTAIRE-1 constructs, showing the effects on neurite outgrowth.