Fig. 8. Nuclear targeting of PCTAIRE-1 abolishes its effects on neurite outgrowth. (A) Indicated constructs were transfected into Neuro-2A cells together with a CD2 marker construct. Two days after transfection, the individual wells were treated with 10 µM forskolin and left for another 24 hours. The wells were incubated for 5 minutes using a Cy3 coupled CD2 antibody, and scored for green/red cells that had neurites longer than twice the length of the cell body. (B) Images of the transfected cells showing the effects on neurite outgrowth.