Fig. 10. Immunostaining of cytoskeletal elements in HEK293 cells co-expressing YFP-ERM and myc-TRPC4. HEK293 cells transiently expressing myc-TRPC4 together with YFP-ERM were fixed with PFA, permeabilized with triton, stained with either an anti-ß-tubulin antibody and then with an Alexa 594-conjugated anti-mouse secondary antibody (A-C) or with phalloidin conjugated to Texas Red (D-F). Images were acquired with a 100x oil immersion objective and filter sets for FITC and Texas Red. YFP-ERM (A,D) and either ß-tubulin (B) or Factin (E) are overlaid in the panels on the right. Bar, 20 µm.