Fig. 2. Subcellular distribution of YFP-EBP50 in human JEG-3 cells. (A,B) Human JEG-3 cells, grown on coverslips, were fixed with PFA, permeabilized with triton and probed with antibodies directed against either ezrin (A) or NHERF/EBP50 (B). The cells were then stained with a secondary antibody conjugated to either Alexa 594 (A) or Alexa 488 (B). (C,D) JEG-3 cells transiently expressing YFP-EBP50 (C) or the YFP alone (D) fixed with PFA. Images were acquired with a 100x oil immersion objective and filter sets for Texas Red (A) and FITC (B-D). Bar, 20 µm.