Fig. 6. Deletion of the ERM-binding domain modifies the subcellular distribution of YFP-EBP50 in human JEG-3 cells. JEG-3 cells transiently expressing YFP-EBP50 (A) or YFP-ERM (C) were fixed with PFA, permeabilized with triton, stained with an anti-ezrin antibody and then with an Alexa 594-conjugated secondary antibody. Images were acquired with a 100x oil immersion objective and filter sets for FITC (A,C) and Texas Red (B,D). Bar, 20 µm.