Fig. 9. Localization of myc-TRPC4 and myc-TRL in HEK293 cells expressing YFP-ERM. HEK293 cells expressing YFP-ERM together with myc-TRPC4 (upper panels) or myc-TRL (lower panels) were fixed with PFA, permeabilized with triton, stained with an anti-myc antibody and then with an Alexa 594-conjugated antimouse secondary antibody. Images were acquired with a 100x oil immersion objective and filter sets for Texas Red [to detect myc-TRPC4 (A) and myc-TRL (C)] and for FITC [to detect YFP-ERM (B,D)]. Bar, 20 µm.