Fig. 1. Effects of LTD₄, CNF-1 and C3 exoenzyme on stress-fibre formation. Int 407 cells were incubated with or without LTD₄, C3 exozyme or CNF-1. The cells were subsequently fixed, permeablised, stained for F-actin with Alexa 488 phalloidin and examined by confocal microscopy. The upper two panels show unstimulated (Control) and LTD₄-treated cells. The two panels in the middle (C3) illustrate cells incubated with 5 µg/ml C3 exoenzyme and 5 µg/ml lipofectamine for 10 hours in the absence or presence of LTD₄. The lower two panels (CNF-1) show cells incubated with 300 ng/ml CNF-1 for 16 hours in the absence or presence of LTD₄. The results illustrated in this figure are representative of ten separate experiments.