Fig. 2. Effects of L63-RhoA and N19-RhoA on stress-fibre formation. (A) Cells were transfected with EGFP-L63-RhoA and then fixed, permeablised, stained for F-actin with Alexa 546 phalloidin and examined by confocal microscopy. (B) Cells were transfected with EGFP-N19-RhoA in the absence or presence of 40 nM LTD₄ for 5 minutes and then processed as in A. Merged images are shown on the right. The results illustrated in this figure are representative of six separate experiments.