Fig. 3. Effects of LTD₄ and TPA on stress-fibre formation. Following stimulation with either 40 nM LTD₄ for 5 minutes or 100 nM TPA for 15 minutes, the cells were fixed, permeablised, stained for F-actin with Alexa 488 phalloidin and examined by confocal microscopy. The different panels show cells that were not stimulated (control) or stimulated with LTD₄ or TPA and also were or were not exposed to 2µm GF109203X or 2 µm Gö6976 for 15 minutes. The results illustrated in this figure are representative of five separate experiments.